Skip to main content
. 1998 Jun;36(6):1653–1659. doi: 10.1128/jcm.36.6.1653-1659.1998

TABLE 1.

Evaluation of the different experimental parameters as applied in the different participating laboratories

Center identification Electrophoresis supply Gels per year Imaging software Run time (h) Voltage (V/cm) Pulsing protocol Temperature (°C) Gel percentage
1 CHEF DR-II 200 Bio Image Advanced Q. 2.01 20 6 20 h: 5.3–34.9" 14 1.0
2 CHEF DR-II 40–50 GelCompar 3.1 25 6 10 h: 5.0–15.0"; 15 h: 15.0–45.0" 14 1.0
3 CHEF DR-III 35 In-house software 26 6 7 h: 5.0–15.0"; 19 h: 15–60" 14 1.0
4 CHEF DR-II 500 GelCompar 4.0 48 6 48 h: 1.0–80.0" 12 1.2
5 CHEF DR-II 1000 Taxotron 20 6 10 h: 10.0"; 10 h: 25.0" 10 0.8
6 Pulsaphor TM 15 In-house software 27 235c 4 h: 5.0"; 6 h: 15.0"; 8 h: 25.0"; 9 h: 35.0" 12 1.2
7 Gene Navigator 100 Taxotron 24 7 24 h: 20–80" 10 1.0
8 CHEF Mapper 40 GelCompar 4.0 20 6 10 h: 5.0–15.0"; 10 h: 15.0–45.0" 14 1.0
9 CHEF DR-II 150–200 Molecular Analyst 20 6 20 h: 1.0–40.0" 12 1.0
10 CHEF DR-II 100 Molecular Analyst 22 6 22 h: 1.0–34.0" 14 0.8
11 CHEF DR-III 200 Biogene 6.32 21 6 2 h: 0.5–5.5"; 18 h: 7.5 to 27.5"; 1 h: 50–65" 14 1.0
12 CHEF DR-II 150 Molecular Analyst 20 6 3 h: 1–10"; 17 h: 5–40" 13 1.4
13a Optional NAb Optional 20 6 20 h: 5.3–34.9" 14 1
a

The parameters for center 13 show the settings for the standardized protocol. Exact size of the gel not reported. 

b

NA, not applicable. 

c

The exact size of the gel was not reported, so the result is given as overall voltage.