TABLE 1.
Evaluation of the different experimental parameters as applied in the different participating laboratories
Center identification | Electrophoresis supply | Gels per year | Imaging software | Run time (h) | Voltage (V/cm) | Pulsing protocol | Temperature (°C) | Gel percentage |
---|---|---|---|---|---|---|---|---|
1 | CHEF DR-II | 200 | Bio Image Advanced Q. 2.01 | 20 | 6 | 20 h: 5.3–34.9" | 14 | 1.0 |
2 | CHEF DR-II | 40–50 | GelCompar 3.1 | 25 | 6 | 10 h: 5.0–15.0"; 15 h: 15.0–45.0" | 14 | 1.0 |
3 | CHEF DR-III | 35 | In-house software | 26 | 6 | 7 h: 5.0–15.0"; 19 h: 15–60" | 14 | 1.0 |
4 | CHEF DR-II | 500 | GelCompar 4.0 | 48 | 6 | 48 h: 1.0–80.0" | 12 | 1.2 |
5 | CHEF DR-II | 1000 | Taxotron | 20 | 6 | 10 h: 10.0"; 10 h: 25.0" | 10 | 0.8 |
6 | Pulsaphor TM | 15 | In-house software | 27 | 235c | 4 h: 5.0"; 6 h: 15.0"; 8 h: 25.0"; 9 h: 35.0" | 12 | 1.2 |
7 | Gene Navigator | 100 | Taxotron | 24 | 7 | 24 h: 20–80" | 10 | 1.0 |
8 | CHEF Mapper | 40 | GelCompar 4.0 | 20 | 6 | 10 h: 5.0–15.0"; 10 h: 15.0–45.0" | 14 | 1.0 |
9 | CHEF DR-II | 150–200 | Molecular Analyst | 20 | 6 | 20 h: 1.0–40.0" | 12 | 1.0 |
10 | CHEF DR-II | 100 | Molecular Analyst | 22 | 6 | 22 h: 1.0–34.0" | 14 | 0.8 |
11 | CHEF DR-III | 200 | Biogene 6.32 | 21 | 6 | 2 h: 0.5–5.5"; 18 h: 7.5 to 27.5"; 1 h: 50–65" | 14 | 1.0 |
12 | CHEF DR-II | 150 | Molecular Analyst | 20 | 6 | 3 h: 1–10"; 17 h: 5–40" | 13 | 1.4 |
13a | Optional | NAb | Optional | 20 | 6 | 20 h: 5.3–34.9" | 14 | 1 |
The parameters for center 13 show the settings for the standardized protocol. Exact size of the gel not reported.
NA, not applicable.
The exact size of the gel was not reported, so the result is given as overall voltage.