TABLE 3.
Evaluation of the experimental data obtained by PFGE of MRSA: survey of molecular size valuesa
MRSA strain | Center 1
|
Center 2
|
Center 3
|
Center 4
|
Center 5
|
Center 6
|
Center 7
|
Center 8
|
Center 10
|
Center 11
|
Center 12
|
Avg value
|
Avg value
|
||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
IH | GP | IH | GP | IH | GP | IH | IH | IH | IH | GP | IH | GP | IH | GP | GP | IH | IH (SD) | GP (SD) | |
1 | 2,813 | 2,679 | 2,846 | 2,829 | 2,763 | 2,788 | 2,848 | 2,416 | 2,813 | 2,924 | 2,400 | 2,792 | 2,932 | 2,750 | 2,550 | 2,702 | 2,710 | 2,768 (130) | 2,697 (165) |
2 | 2,813 | 2,679 | 2,828 | 2,812 | 2,779 | 2,787 | 2,856 | 2,416 | 2,813 | 2,787 | 2,387 | 2,792 | 2,932 | 2,700 | 2,580 | 2,725 | 2,716 | 2,750 (120) | 2,700 (164) |
3 | 2,813 | 2,679 | 2,785 | 2,812 | 2,785 | 2,783 | 2,874 | 2,416 | 2,813 | 2,819 | 2,468 | 2,792 | 2,832 | 2,700 | 2,570 | 2,665 | 2,704 | 2,750 (122) | 2,687 (124) |
4 | 2,813 | 2,679 | 2,818 | 2,881 | 2,786 | 2,784 | 2,854 | 2,416 | 2,813 | 2,763 | 2,467 | 2,792 | 2,932 | 2,700 | 2,550 | 2,673 | 2,719 | 2,747 (119) | 2,709 (156) |
5 | 2,813 | 2,679 | 2,796 | 2,818 | 2,770 | 2,783 | 2,881 | 2,416 | 2,813 | 2,932 | 2,494 | 2,792 | 2,932 | 2,700 | 2,610 | 2,644 | 2,720 | 2,763 (133) | 2,709 (135) |
6 | 2,227 | 2,068 | 2,153 | 2,378 | 2,452 | 2,173 | 2,481 | 1,943 | 2,402 | 2,299 | 2,076 | 2,286 | 2,265 | 2,010 | 2,020 | 2,222 | 2,133 | 2,239 (172) | 2,171 (118) |
7 | 2,247 | 2,078 | 2,090 | 2,301 | 2,460 | 2,180 | 2,540 | 1,768 | 2,243 | 2,277 | 2,045 | 2,207 | 2,179 | 2,010 | 2,050 | 2,077 | 2,158 | 2,200 (207) | 2,130 (87) |
8 | 2,412 | 2,238 | 2,129 | 2,317 | 2,594 | 2,347 | 2,673 | 1,960 | 2,378 | 2,461 | 2,226 | 2,406 | 2,460 | 2,280 | 2,220 | 2,194 | 2,300 | 2,359 (198) | 2,286 (88) |
9 | 2,400 | 2,228 | 2,111 | 2,322 | 2,611 | 2,343 | 2,681 | 2,093 | 2,383 | 2,465 | 2,189 | 2,406 | 2,449 | 2,310 | 2,220 | 2,749 | 2,283 | 2,374 (179) | 2,357 (180) |
10 | 2,370 | 2,230 | 2,104 | 2,362 | 2,616 | 2,331 | 2,656 | 1,905 | 2,383 | 2,483 | 2,206 | 2,342 | 2,435 | 2,280 | 2,230 | 2,155 | 2,256 | 2,340 (213) | 2,278 (92) |
11 | 2,593 | 2,673 | 2,811 | 2,721 | 2,675 | 2,627 | 2,567 | 2,603 | 2,690 | 3,431 | 2,456 | 2,513 | 2,677 | 2,770 | 2,680 | 2,447 | 2,476 | 2,713 (260) | 2,612 (104) |
12 | 2,912 | 2,705 | 2,701 | 2,759 | 2,787 | 2,768 | 2,854 | 1,860 | 2,931 | 2,657 | 2,358 | 2,950 | 3,299 | 2,770 | 2,830 | 2,780 | 2,714 (317) | 2,786 (255) | |
13 | 2,586 | 2,483 | 2,670 | 2,701 | 2,547 | 2,541 | 2,431 | 2,125 | 2,716 | 3,023 | 2,499 | 2,403 | 2,571 | 3,080 | 2,580 | 2,464 | 2,278 | 2,586 (287) | 2,548 (74) |
14 | 2,892 | 2,752 | 2,954 | 3,052 | 2,731 | 2,753 | 2,776 | 2,371 | 2,867 | 2,360 | 2,969 | 2,674 | 2,841 | 2,900 | 2,650 | 2,694 | 2,706 | 2,705 (193) | 2,816 (136) |
15 | 2,672 | 2,470 | 2,725 | 2,790 | 2,516 | 2,491 | 2,542 | 2,080 | 2,536 | 2,540 | 2,574 | 2,464 | 2,596 | 2,400 | 2,680 | 2,502 | 2,539 | 2,501 (166) | 2,586 (107) |
16 | 2,735 | 2,544 | 2,807 | 2,726 | 2,141 | 2,587 | 2,708 | 2,531 | 2,929 | 2,821 | 2,327 | 2,631 | 2,709 | 2,810 | 2,650 | 2,805 | 2,708 | 2,682 (208) | 2,628 (154) |
17 | 2,514 | 2,307 | 2,788 | 2,833 | 2,434 | 2,396 | 2,787 | 1,940 | 2,621 | 2,770 | 2,133 | 2,296 | 2,733 | 2,330 | 2,410 | 2,282 | 2,104 | 2,458 (279) | 2,442 (233) |
18 | 3,929 | 2,794 | 2,766 | 2,817 | 2,407 | 2,709 | 2,471 | 2,192 | 2,799 | 2,645 | 2,432 | 2,572 | 2,722 | 2,710 | 2,830 | 3,107 | 2,613 | 2,710 (441) | 2,773 (185) |
19 | 1,909 | 1,737 | 2,479 | 2,593 | 2,527 | 2,441 | 2,612 | 1,402 | 1,878 | 2,726 | 1,497 | 1,656 | 1,846 | 1,800 | 1,870 | 2,263 | 2,542 | 2,153 (448) | 2,035 (372) |
20 | 2,404 | 1,925 | 2,633 | 2,133 | 2,770 | 2,785 | 2,820 | 3,077 | 2,671 | 2,966 | 1,947 | 2,577 | 2,349 | 2,210 | 2,860 | 2,117 | 2,681 (253) | 2,302 (354) |
The molecular length values are given in kilobase pairs. Centers 9 and 11 experienced some difficulties with efficient normalization and band recognition by the available software, hence, the absence of numerical data and the discrepancy of chromosome sizes for the identical isolates 1 to 5. For centers 4, 5, 6, and 11, the GP protocol did not give rise to interpretable results. Center 4 did not perform the GP analysis. SD, standard deviation. The average values are given per strain. When groups of isolates are considered, the following general averages were calculated: isolates 1 to 5, 2,756 (IH) and 2,700 (GP); isolates 6 to 10, 2,302 (IH) and 2,244 (GP); isolates 11 to 20, 2,590 (IH) and 2,552 (GP); IH, in-house protocol.