TABLE 4.
Evaluation of the experimental data obtained by PFGE of MRSA: pattern similarity (mean values) and range of the total number of bands per strain by epidemiological group
Center identification | In-house procedure
|
Standardized procedure
|
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Identical isolates (1 to 5)
|
Clustered isolates (6–10)
|
Independent isolates (11 to 20)
|
Identical isolates (1 to 5)
|
Clustered isolates (6 to 10)
|
Independent isolates (11 to 20)
|
|||||||
% | na | % | n | % | n | % | n | % | n | % | n | |
1 | 100 | 15 | 96 | 15–16 | 64 | 13–16 | 100 | 15 | 92 | 14–16 | 53 | 12–15 |
2 | 100 | 16 | 87 | 15–16 | 26 | 13–16 | 98 | 15 | 89 | 14–15 | 31 | 11–16 |
3 | 100 | 15 | 93 | 15–16 | 67 | 12–16 | 96 | 13 | 92 | 13–14 | 59 | 11–15 |
4 | 100 | 16 | 88 | 16–17 | 27 | 13–18 | —b | — | — | — | — | — |
5 | 100 | 15 | 93 | 14–15 | 38 | 10–15 | — | — | — | — | — | — |
6 | 100 | 16 | 89 | 16–17 | 22 | 14–18 | — | — | — | — | — | — |
7 | 100 | 10–12 | 94 | 9–10 | 30 | 8–12 | 98 | 12 | 87 | 11–12 | 55 | 10–14 |
8 | 100 | 16 | 87 | 16–17 | 34 | 12–17 | 92 | 16 | 62 | 17 | 31 | 14–18 |
9 | 98 | 16 | 79 | 13–14 | 52 | 13–16 | 94 | 14 | 94 | 13 | 67 | 12–14 |
10 | 100 | 16 | 68 | 14–16 | 30 | 11–18 | 100 | 15 | 85 | 14 | 44 | 10–15 |
11 | 100 | 15 | — | 14–16 | — | 12–18 | 100 | 15 | — | 15–17 | — | 11–18 |
12 | 100 | 17 | 85 | 16–17 | 49 | 16–18 | — | — | — | — | — | — |
Overall range (no. of fragment) | 10–17 | 9–17 | 8–18 | 12–16 | 11–17 | 10–18 | ||||||
Avg | 99.8 | 87.2 | 39.9 | 97.3 | 85.9 | 48.6 | ||||||
SD | 0.6 | 7.6 | 15.0 | 2.8 | 10.2 | 12.8 |
n: number of bands.
—, no data or nonevaluable data.