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Primer sequences used for multiplex PCR
| Name | Function | Sequence | Primer size (bp) | Product size (bp) |
|---|---|---|---|---|
| A | Forward primer (biosynthesis region) | 5′-TTTATCACTATCACCGTCCACACCT-3′ | 25 | 1,114 |
| B | Reverse primer (biosynthesis region) | 5′-CATTCGGGTCTTGTGGCTACTAAA-3′ | 23 | |
| C | Forward primer (export region) | 5′-TGGCGATACCGGAAACAGAGTC-3′ | 22 | 715 |
| D | Reverse primer (export region) | 5′-GCGAAAGGCTATGGTATGGGTATGG-3′ | 24 |
