TABLE 2.
Protocols for brucellosis agglutination tests
| Test | Serum (μl) | Antigen (μl) | Test surface | Incubation | Agitation |
|---|---|---|---|---|---|
| SATA | 80, 40, 20, 10, 5 | 2,000 | Glass tube | 48 h @ 37°C | Mix once at beginning |
| SATM | 80, 40, 20, 10, 5 | 2,000 | Glass tube | 48 h @ 37°C | Mix once at beginning |
| SPT | 80, 40, 20, 10 | 30 | Glass plate | 8 min in box | Swirl 4× pre, mid, post |
| BAPA | 80 | 30 | Glass plate | 8 min in box | Swirl 4× pre, mid, post |
| 8%Card | 30 | 30 | Opaque paper card | 4 min | Rock 16×/mina |
| RAP | 30 | 20 | Microtiter plate | 30 min | Rotate first 10 mina |
| 8%RB | 30 | 30 | Glass plate | 4 min | Rock 16×/min |
| 3%RB | 30 | 30 | Glass plate | 4 min | Rock 16×/min |
| 4.5%RB | 30 | 30 | Opaque plastic plate | 4 min | Rotatea |
| URIV | 80, 40, 20, 10b | 30 | Glass plate | 12 min in box | Swirl 4× pre, mid, post |
| MRIV | 80, 40, 20, 10b | 30 | Glass plate | 12 min in box | Swirl 4× pre, mid, post |
a
Mechanical agitation.
b
Serum was pretreated by dilution and a standard plate test was performed with equal amounts of 1% rivanol solution (supernatant used).