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. 2023 Sep 8;18(9):e0291414. doi: 10.1371/journal.pone.0291414

Table 4. Summary of students’ previous knowledge regarding public health topics: (A) Did not know about the topic; (B) Did not learn about the topic in classes.

(A) Did not know about the topic (B) Did not learn about it in classes
Total (N = 545) Sex (%) p Total (N = 545) Sex (%) P
Male (N = 192) Female (N = 353) Male (N = 192) Female (N = 353)
Nutrition and Physical Activity
Lifestyle-related diseases 46 (8.4) 19 (9.9) 27 (7.6) .348 11 (2) 7 (3.6) 4 (1.1) .044
Food poisoning 38 (7) 15 (7.8) 23 (6.5) .570 21 (3.9) 10 (5.2) 11 (3.1) .225
Allergic reaction 8 (1.5) 6 (3.1) 2 (0.6) .018 7 (1.3) 5 (2.6) 2 (0.6) .044
Alcohol, Tobacco, and Other Drugs
Health effect of smoking 1 (0.2) 1 (0.5) 0 (0) NA 0 (0) 0 (0) 0 (0) NA
Health hazard of passive smoking 10 (1.8) 1 (0.5) 9 (2.5) .092 5 (0.9) 1 (0.5) 4 (1.1) .475
Smoking effect on pregnancy 31 (5.7) 15 (7.8) 16 (4.5) .114 21 (3.9) 8 (4.2) 13 (3.7) .779
Dependence of smoking 6 (1.1) 1 (0.5) 5 (1.4) .341 2 (0.4) 0 (0) 2 (0.6)
Health effect of e-cigarettes 197 (36.1) 63 (32.8) 134 (38) .223 175 (32.1) 58 (30.2) 117 (33.1) .470
Effect of alcohol consumption 9 (1.7) 4 (2.1) 5 (1.4) .554 4 (0.7) 2 (1) 2 (0.6) .531
Alcohol consumption effect on pregnancy 39 (7.2) 20 (10.4) 19 (5.4) .030 24 (4.4) 11 (5.7) 13 (3.7) .269
Mental/physical effect of mind-altering drugs 1 (0.2) 1 (0.5) 0 (0) NA 1 (0.2) 1 (0.5) 0 (0) NA
Alcohol, smoking and drug addiction 33 (6.1) 12 (6.3) 21 (5.9) .888 17 (3.1) 7 (3.6) 10 (2.8) .602
Addiction 117 (21.5) 45 (23.4) 72 (20.4) .418 85 (15.6) 31 (16.1) 54 (15.3) .805
Growth, Development and Sexual Health
Ovulation and menstruation 17 (3.1) 15 (7.8) 2 (0.6) < .001 8 (1.5) 7 (3.6) 1 (0.3) .002
Ejaculation 21 (3.9) 1 (0.5) 20 (5.7) .003 10 (1.8) 1 (0.5) 9 (2.5) .094
How to use condoms 129 (23.7) 15 (7.8) 114 (32.3) < .001 86 (15.8) 10 (5.2) 76 (21.5) < .001
Woman’s basal body temperature 190 (34.9) 103 (53.6) 87 (24.6) < .001 139 (25.5) 76 (39.6) 63 (17.8) < .001
Measurement of woman’s basal body temperature 274 (50.3) 128 (66.7) 146 (41.4) < .001 217 (39.8) 106 (55.2) 111 (31.4) < .001
Fertilization and pregnancy 7 (1.3) 3 (1.6) 4 (1.1) .666 2 (0.4) 1 (0.5) 1 (0.3) .659
Gestation period and childbirth 67 (12.3) 30 (15.6) 37 (10.5) .085 24 (4.4) 15 (7.8) 9 (2.5) .004
Public services related to pregnancy 96 (17.6) 44 (22.9) 52 (14.7) .015 63 (11.6) 32 (16.7) 31 (8.8) .006
Significance and importance of family planning 155 (28.4) 60 (31.3) 95 (26.9) .293 118 (21.7) 43 (22.4) 75 (21.2) .768
Abortion under Maternal Health Act in Japan 98 (18) 46 (24) 52 (14.7) .008 67 (12.3) 35 (18.2) 32 (9.1) .002
Sex and gender 131 (24) 33 (17.2) 98 (27.8) .005 105 (19.3) 27 (14.1) 78 (22.1) .022
LGBTQIA+ 118 (21.7) 41 (21.4) 77 (21.8) .862 96 (17.6) 33 (17.2) 63 (17.8) .813
Personal and Community Health
Birth control pills and preventing sexually transmitted diseases 206 (37.8) 73 (38) 133 (37.7) .940 163 (29.9) 58 (30.2) 105 (29.7) .913
Prevention of sexually transmitted diseases 28 (5.1) 12 (6.3) 16 (4.5) .383 16 (2.9) 8 (4.2) 8 (2.3) .208
Vaccines 26 (4.8) 11 (5.7) 15 (4.2) .428 21 (3.9) 9 (4.7) 12 (3.4) .445

NOTE: Values in parenthesis represent percentage of valid N for each category. Results are based on two-sided tests for comparison between sexes. The p-value threshold for significance was adjusted for multiple comparisons using the Bonferroni correction. The threshold for statistical significance was set at p < .05 (S1 File).