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[Preprint]. 2023 Oct 28:2023.06.12.544638. [Version 3] doi: 10.1101/2023.06.12.544638

Figure 6. Exogenous FBD1 fragment of MYCBP2 disrupts EPHB2-MYCBP2 binding and impairs EPHB2 function in HeLa cells.

Figure 6.

A) Schematic illustrating competition of exogenous MYCBP2-FBD1 fragment that disrupts MYCBP2-FBXO45 binding and leads to MYCBP2 reduction in EPHB2 complexes. B) Exogenous FBD1 WT overexpression leads to reduced EPHB2-MYCBP2 binding in HEK293 cells despite co-expression of FBXO45. C) FBD1 overexpression also disrupts EPHB2-MYCBP2 binding in the absence of FBXO45 overexpression. D) Representative images of ephrin-B2 stripe assays using HeLa cells expressing GFP-FBD1 mut or GFP-FBD1 WT. E) Quantification of cells present on eB2 stripes (p=0.0107, two-tailed unpaired t-test). Error bars represent SD.