TABLE 3.
Discordant duplicate PCR results by the AMPLICOR test with archived CSF specimens
| CSF specimen no. | AMPLICOR result(s) (OD450)a
|
In-house RT-PCR result
|
Cell culture result | |||
|---|---|---|---|---|---|---|
| 1st assay | 2nd assay | RMSb | 1st assay | 2nd assay | ||
| 1 | 0.137, 0.414 | 0.198, 0.917 | 0.726 | − | + | − |
| 2 | 0.175, 0.798 | 0.213, 0.987 | 2.824 | + | + | − |
| 3 | 0.161, 0.332 | 0.158, 0.580 | 0.105 | + | − | Echo-9 |
| 4 | 0.133, 0.135 | 0.155, 0.346 | 0.950 | − | − | Echo-9 |
| 5 | 0.131, 0.544 | 0.151, 0.484 | 0.061 | + | + | − |
| 6 | 0.129, 0.518 | 0.159, 0.330 | 1.234 | + | + | − |
| 7 | 0.110, 0.127 | 0.184, 0.604 | 0.053 | + | + | Echo-30 |
| 8 | 0.187, 0.521 | 0.156, 0.411 | 3.008 | + | + | − |
a
Positive, >0.500; indeterminate, 0.250 to 0.500; negative, <0.250. Each specimen was assayed in duplicate (two microwell plate results) in the first and second assays with the AMPLICOR test.
b
Results obtained by Roche Molecular Systems, Somerville, N.J.