Table 1: plasmids.
HUGO Gene names and protein numbering are indicated. Human sequences were used unless a species is indicated (except for fluorescent proteins).
| Plasmid | Backbone | Insert | Reference |
|---|---|---|---|
| NES-EGFP-P4Mx1 | pEGFP-C1 | X.leavis map2k1.L(32-44):EGFP:L. pneumophila SidM546-647 | (Zewe et al., 2018) |
| EGFP-P4Mx2 | pEGFP-C1 | EGFP:L. pneumophila SidM(546-647):SidM546-647 | (Hammond et al., 2014) |
| mCherry-P4Mx2 | pEGFP-C1 | mCherry:L. pneumophila SidM(546-647):SidM546-647 | (Hammond et al., 2014) |
| EGFP-P4C | pEGFP-C1 | EGFP:L.pneumophila SidC(608-773) | (Weber et al., 2014) |
| mRFP-P4C | pEGFP-C1 | mRFP:L.pneumophila SidC(608-773) | (Weber et al., 2014) |
| iRFP713-PH-PLCδ1 | piRFP-C1 | iRFP713:Rattus norvegicus Plcd110-140 | (Idevall-Hagren et al., 2012) |
| EGFP-MAPPER | pEGFP-C1 | EGFP:MAPPER | (Chang et al., 2013) |
| neonGreen-ORP2 | pNeonGreen-C1 | neonGreen:ORP2 | This study |
| neonGreen-ORP3 | pNeonGreen-C1 | neonGreen:ORP3 | This study |
| neonGreen-ORP5 | pNeonGreen-C1 | neonGreen:ORP5 | This study |
| neonGreen-ORP6 | pNeonGreen-C1 | neonGreen:ORP6 | This study |
| mCherry-VAPB | pmCherry-C1 | mCherry-VAPB | (Zewe et al., 2018) |
| mCherry-ORP5 | pmCherry-C1 | mCherry:OSBPL5(isoform a) | (Sohn et al., 2018) |
| mCherry-ORP5ΔTMD-FKBP | pmCherry-C1 | mCherry:OSBPL5(isoform a)1-856:FKBP3-108 | This study |
| mCherry-ORP5ΔORDΔTMD-FKBP | pmCherry-C1 | mCherry:OSBPL5(isoform a)1-140-SGG-OSBPL5(isoform a)735-856-856:FKBP3-108 | This study |
| mCherry-ORP5H478/9A-ΔTMD-FKBP | pmCherry-C1 | mCherry:OSBPL5(isoform a)1-856H478/479A:FKBP3-108 | This study |
| mCherry-FKBP-ELL-CAAX | pmCherry-C1 | mCherry:SA(GGSA)4-RAALG:FKBP3-108:SA(GGSA)4PR(GGS)5GG-RAQ-(GGSGG)2-(EAAR)5-(GGSGG)2-SNSAVD-HRAS172-189 | This study |
| Akap1N31-FRB-iRFP | piRFP-N1 | Mus musculus Akap11-31-M16L:MTOR2021-2113:iRFP | (Zewe et al., 2020) |
| iRFP-FRB-ER | piRFP-C1 | iRFP: MTOR2021-2113:[GGSA]2ILNSRV:SACM1L(521-587) | (Zewe et al., 2020) |
| COX8AN29x2-mCherry | pmCherry-N1 | COX8A1-29:mCherry | (Zewe et al., 2020) |
| EGFP-SAC1mito | pEGFP-C1 | EGFP:SACM1L1-521:FIS1122-152 | This study |
| TagBFP2-SAC1mito | pTagBFP2-C1 | TagBFP2:SACM1L1-521:FIS1122-152 | This study |
| TagBFP2-SAC1mito-C389S | pTagBFP2-C1 | TagBFP2:SACM1L1-521C389S:FIS1122-152 | This study |
| iRFP-FRB-mito | piRFP-C1 | iRFP: MTOR2021-2113:[GGSA]2 QASNSAVDGTA:FIS1122-152 | This study |
| mCherry-FKBP-PI4KAC1001 | pmCherry-C1 | mCherry: TagBFP2: FKBP1A3-108:[GGSA]4GG:PI4KA1102-2103 | (Zewe et al., 2020) |