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[Preprint]. 2023 Aug 29:2023.08.28.555135. [Version 1] doi: 10.1101/2023.08.28.555135

Table 2. Microglial ultrastructural features in CD versus KD fed mice exposed to RSD.

CD: control diet; KD: ketogenic diet; RSD: repeated social defeat.

Ultrastructural analysis
mean ± standard error of the mean F P
Control Susceptible Resistant
CD KD CD KD CD KD
Contacts with other cells Blood vessels 0.264 ± 0.077 0.171 ± 0.065 0.182 ± 0.068 0.222 ± 0.070 0.079 ± 0.044 0.243 ± 0.072 F(2, 206) Stress x diet 1.992 0.139
F(2, 206) Stress 0.3478 0.7066
F(1, 206) Diet 0.5246 0.4697
Astrocytes 0.088 ± 0.049 0.057 ± 0.040 0.091 ± 0.051 0.194 ± 0.067 0 0.194 ± 0.067 F(2, 206) Stress x diet 1.393 0.2506
F(2, 206) Stress 1.864 0.1576
F(1, 206) Diet 2.466 0.1179
Neurons 0.177 ± 0.099 0.086 ± 0.048 0.121 ± 0.072 0.028 ± 0.028 0.121 ± 0.072 0.108 ± 0.052 F (2, 206) Stress x diet 0.4356 0.6475
F (2, 206) Stress 0.4217 0.6565
F (1, 206) Diet 1.41 0.2364
Mitochondria Standard 3 ± 0.431 2.571 ± 0.383 1.424 ± 0.275 1.972 ± 0.254 2.421 ± 0.319 2.243 ± 0.348 F (2, 206) Stress x diet 1.076 0.3429
F (2, 206) Stress 5.003 0.007**
F (1, 206) Diet 0.003 0.95
Long 0.205 ± 0.082 0.114 ± 0.068 0.091 ± 0.667 0 0.263 ± 0.134 0.054 ± 0.054 F (2, 206) Stress x diet 0.3563 0.7007
F (2, 206) Stress 1.327 0.2675
F (1, 206) Diet 3.913 0.0493*
Total healthy 3.206 ± 0.446 2.686 ± 0.399 1.515 ± 0.292 1.972 ± 0.254 2.684 ± 0.392 2.297 ± 0.359 F (2, 206) Stress x diet 1.025 0.3606
F (2, 206) Stress 5.391 0.0052**
F (1, 206) Diet 0.243 0.6226
Dystrophic 0.677 ± 0.201 0.6 ± 0.170 0.485 ± 0.139 0.417 ± 0.122 0.29 ± 0.130 0.351 ± 0.111 F (2, 206) Stress x diet 0.162 0.8506
F (2, 206) Stress 2.285 0.1044
F (1, 206) Diet 0.0406 0.8405
White 0.823 ± 0.251 0.8 ± 0.158 0.485 ± 0.180 0.389 ± 0.115 0.29 ± 0.092 0.838 ± 0.184 F (2, 206) Stress x diet 2.097 0.1254
F (2, 206) Stress 2.754 0.066
F (1, 207) Diet 1.222 0.2703
Long and dystrophic 0.088 ± 0.049 0.057 ± 0.040 0.061 ± 0.061 0 0.053 ± 0.037 0 F (2, 206) Stress x diet 0.0799 0.9233
F (2, 206) Stress 0.9164 0.4016
F (1, 206) Diet 2.426 0.1209
Holy 0.294 ± 0.115 0.114 ± 0.054 0.09 ± 0.050 0 0.105 ± 0.062 0.27 ± 0.107 F (2, 206) Stress x diet 2.503 0.0843
F (2, 206) Stress 2.478 0.0864
F (1, 206) Diet 0.4614 0.4977
Total dystrophic 2 ± 0.347 1.571 ± 0.276 1.545 ± 0.237 0.944 ± 0.173 0.842 ± 0.215 1.514 ± 0.281 F (2, 206) Stress x diet 2.464 0.0876
F (2, 206) Stress 2.012 0.1363
F (1, 206) Diet 0.1194 0.73
% of dystrophic mitochondria 30.55 ± 5.288 38.04 ± 6.159 37.27 ± 6.783 27.54 ± 4.797 19.94 ± 5.166 31.7 ± 5.146 F (2, 206) Stress x diet 1.998 0.1383
F (2, 206) Stress 1.384 0.2529
F (1, 206) Diet 0.4339 0.5108
sy na Axon terminal contacts 7.353 ± 0.973 8.229 ± 0.659 4.727 ± 0.627 5.361 ± 0.707 5.816 ± 0.542 6.378 ± 0.657 F (2, 206) Stress x diet 0.0199 0.9803
F (2, 206) Stress 7.542 0.0007***
F (1, 206) Diet 1.535 0.2168
Dendritic spine contacts 0.588 ± 0.159 0.371 ± 0.101 0.061 ± 0.042 0.222 ± 0.106 0.526 ± 0.118 0.297 ± 0.109 F (2, 206) Stress x diet 1.891 0.1535
F (2, 206) Stress 5.036 0.0073**
F (1, 206) Diet 1.006 0.317
Synaptic cleft contacts 0.912 ± 0.186 1.286 ± 0.227 0.667 ± 0.178 0.417 ± 0.134 0.684 ± 0.161 0.946 ± 0.155 F (2, 206) Stress x diet 1.711 0.1833
F (2, 206) Stress 4.951 0.0079**
F (1, 206) Diet 0.6898 0.4072
Total synaptic contacts 8.853 ± 1.145 9.886 ± 0.803 5.455 ± 0.702 6 ± 0.781 7.026 ± 0.631 7.622 ± 0.761 F (2, 206) Stress x diet 0.049 0.9521
F (2, 206) Stress 9.745 <0.0001****
F (1, 206) Diet 1.228 0.2691
Other organelles Primary lysosomes 0.118 ± 0.070 0.343 ± 0.136 0.212 ± 0.113 0.139 ± 0.071 0.158 ± 0.110 0.135 ± 0.069 F (2, 206) Stress x diet 1.4 0.249
F (2, 206) Stress 0.4844 0.6168
F (1, 206) Diet 0.1918 0.6619
Secondary lysosomes 0.118 ± 0.056 0.114 ± 0.068 0 0.028 ± 0.028 0 0.108 ± 0.085 F (2, 206) Stress x diet 0.6626 0.5166
F (2, 206) Stress 0.91 0.1508
F (1, 206) Diet 1.135 0.2881
Tertiary lysosomes 0.294± 0.099 0 0.091± 0.051 0.111± 0.053 0.079 ± 0.044 0.054 ± 0.038 F (2, 206) Stress x diet 4.715 0.0100**
F (2, 206) Stress 1.069 0.3451
F (1, 206) Diet 4.866 0.0285*
Total lysosomes 0.529 ± 0.159 0.457 ± 0.166 0.303 ± 0.119 0.278 ± 0.094 0.237 ± 0.116 0.297 ± 0.109 F (2, 206) Stress x diet 0.0983 0.9064
F (2, 206) Stress 1.943 0.1459
F (1, 206) Diet 0.0319 0.8584
Phagosomes with content 0.265 ± 0.114 0.343 ± 0.100 0.121 ± 0.072 0.5 ± 0.180 0.447 ± 0.167 0.378 ± 0.098 F (2, 206) Stress x diet 1.627 0.1991
F (2, 206) Stress 0.3757 0.6873
F (1, 206) Diet 1.334 0.2495
Phagosomes with myelin 0.324 ± 0.173 0.286 ± 0.145 0.061 ± 0.042 0.167 ± 0.075 0.079 ± 0.044 0.027 ± 0.027 F (2, 206) Stress x diet 0.3845 0.6813
F (2, 206) Stress 3.51 0.0317*
F (1, 206) Diet 0.0049 0.9439
Empty phagosomes 1.265 ± 0.305 0.486 ± 0.126 1.273 ± 0.299 0.75 ± 0.230 1 ± 0.226 0.487 ± 0.184 F (2, 206) Stress x diet 0.1741 0.8403
F (2, 206) Stress 0.7707 0.464
F (1, 206) Diet 10.45 0.0014**
Total phagosomes 1.853 ± 0.489 1.114 ± 0.245 1.455 ± 0.296 1.417 ± 0.366 1.526 ± 0.326 0.833 ± 0.247 F (2, 206) Stress x diet 0.6657 0.515
F (2, 206) Stress 0.4791 0.62
F (1, 206) Diet 3.16 0.0769
Lipofuscin granules 0.471 ± 0.121 0.171 ± 0.087 0.121 ± 0.058 0.306 ± 0.118 0.395 ± 0.153 0.351 ± 0.111 F (2, 206) Stress x diet 2.237 0.1094
F (2, 206) Stress 0.8058 0.4481
F (1, 206) Diet 0.5399 0.4633
Lipid droplets 0.765 ± 0.164 0.629 ± 0.229 0.727 ± 0.236 0.361 ± 0.183 0.447 ± 0.222 0.297 ± 0.109 F (2, 206) Stress x diet 0.222 0.8011
F (2, 206) Stress 1.358 0.2595
F (1, 206) Diet 1.806 0.1805
Standard Golgi apparatus 0.265 ± 0.097 0.514 ± 0.155 0.091 ± 0.067 0.417 ± 0.175 0.263 ± 0.082 0.297 ± 0.128 F (2, 206) Stress x diet 0.8598 0.4248
F (2, 206) Stress 0.6911 0.5022
F (1, 206) Diet 3.715 0.0553
Dystrophic Golgi apparatus 0.353 ± 0.102 0.286 ± 0.112 0.697 ± 2282 0.278 ± 0.116 0.342 ± 0.120 0.139 ± 0.070 F (2, 206) Stress x diet 0.8884 0.4129
F (2, 206) Stress 1.839 0.1616
F (1, 206) Diet 4.571 0.0337*
Proportion dystrophic Golgi apparatus 22.550 x ± 0.070 11.86 ± 5.005 30.3 ± 7.861 15.05 ± 5.768 22.37 ± 6.450 8.333 ± 4.033 F (2, 206) Stress x diet 0.0927 0.9115
F (2, 206) Stress 0.899 0.4086
F (1, 206) Diet 7.724 0.0060**
Nuclear pores 0.76 ± 0.270 1.2 ± 0.306 1.485 ± 0.547 1.667 ± 0.384 1.132 ± 0.267 0.865 ± 0.187 F (2, 206) Stress x diet 0.4772 0.6212
F (2, 206) Stress 1.862 0.158
F (1, 206) Diet 0.1713 0.6794
Nuclear indentations 0.177 ± 0.066 0.2 ± 0.090 0.03 ± 0.030 0.167 ± 0.075 0.079 ± 0.044 0.054 ± 0.038 F (2, 206) Stress x diet 0.9027 0.4071
F (2, 206) Stress 2.111 0.1237
F (1, 206) Diet 0.8304 0.3632
Nuclear alterations 0.2941 ± 0.0992 0.3429 ± 0.0999 0.2424 ± 0.1308 0.2222 ± 0.0807 0.1316 ± 0.0555 0.1389 ± 0.0707 F (2, 206) Stress x diet 0.0714 0.9311
F (2, 206) Stress 2.068 0.1291
F (1, 206) Diet 0.0259 0.8721
Total nuclear alterations 0.677 ± 0.304 0.543 ± 0.150 0.273 ± 0.140 0.389 ± 0.134 0.211 ± 0.077 0.189 ± 0.094 F (2, 206) Stress x diet 0.1523 0.8589
F (2, 206) Stress 3.102 0.0471*
F (1, 206) Diet 0.3263 0.5685
Standard endoplasmic reticulum 7.088 ± 0.875 6.029 ± 0.885 3.303 ± 0.466 5.139 ± 0.930 6.158 ± 0.931 4.838 ± 0.745 F (2, 206) Stress x diet 2.161 0.1178
F (2, 206) Stress 3.84 0.0230*
F (1, 206) Diet 0.0647 0.7994
Dilated endoplasmic reticulum 1.559 ± 0.401 0.486 ± 0.144 0.901 ± 0.315 0.389 ± 0.115 0.711 ± 0.346 0.541 ± 0.158 F (2, 206) Stress x diet 1.483 0.2293
F (2, 206) Stress 1.325 0.2681
F (1, 206) Diet 7.02 0.0087**
Endoplasmic reticulum with content 0.353 ± 0.242 0.114 ± 0.055 0.091 ± 0.051 0 0 0.081 ± 0.060 F (2, 206) Stress x diet 1.246 0.2897
F (2, 206) Stress 2.256 0.1073
F (1, 206) Diet 0.9559 0.3294
Total dystrophic endoplasmic reticulum 1.912 ± 0.558 0.6 ± 0.165 1 ± 0.323 0.389 ± 0.115 0.711 ± 0.346 1.135 ± 0.575 F (2, 206) Stress x diet 1.991 0.1391
F (2, 206) Stress 2.202 0.1132
F (1, 206) Diet 6.766 0.0100**
Proportion dystrophic endoplasmic reticulum 15.72 ± 3.743 10.23 ± 3.369 16.88 ± 4.066 10.68 ± 4.066 7.983 ± 3.386 9.152 ± 3.034 F (2, 206) Stress x diet 0.6525 0.5218
F (2, 206) Stress 1.1 0.3348
F (1, 206) Diet 1.264 0.2622
Autophagosomes 0.206 ± 0.102 0.143 ± 0.073 0.091 ± 0.067 0.083 ± 0.047 0.053 ± 0.037 0.054 ± 0.038 F (2, 206) Stress x diet 0.156 0.8556
F (2, 206) Stress 1.93 0.1477
F (1, 206) Diet 0.1905 0.6629