Figure 7. Phase 2 context fear conditioning in male mice, separated by swim condition.

Male wildtypes are represented by orange solid lines, and male heterozygotes are represented by yellow dashed lines. Fear conditioning occurred 4 weeks after swim stress exposure for Phase 2. Mice were trained on Day 0 in Context A by administering five pseudo-randomized 1 s, 0.8 mA foot shocks following a two minute baseline (A,C). Two days later (Day 2), mice were placed back in Context A to test for context fear expression (B,D). Data are percent time spent freezing for each 30 s period following the foot shock (A,C), or every 30 s of testing (B,D). Male wildtype no swim, n=9; male heterozygous no swim, n=9; male wildtype swim, n=9; male heterozygous swim, n=10. Data are graphed as mean ± 95% confidence interval. *p=0.034 (panel B); *p=0.048, p=0.013 (left to right, panel D); **p=0.004 (panel D); indicate difference between heterozygous and wildtype within same sex at the indicated timepoint. *p=0.030, *p=0.035, *p=0.011 (left to right, panel D); indicate difference between no swim and swim conditions within same sex and genotype (indicated by color) at the indicated timepoint.