Figure 2: Unrestricted naturalistic locomotion behavior with ONIX.

a, Overview of experiment: Mice were freely exploring a 3D arena made out of styrofoam pieces of varying heights. b, Unimplanted mice and mice with a standard tether (top) or ONIX micro coax (bottom) were tracked in 3d using multicamera markerless pose estimation³¹. c, Head yaw and pitch occupancies over the course of a recording. Standard tethering significantly reduces freedom of head movement relative to unimplanted mice, whereas ONIX does not. d, Speed distributions over the course of a recording. Standard implants significantly reduce running speed, while ONIX results in only a modest speed reduction relative to unimplanted mice. e, 2D projection of mouse trajectories over the course of a recording session. ONIX retains the same spontaneous exploration behavior as unimplanted mice, while standard headstages and cables greatly reduce locomotion.