Figure 2.

Overt disease observed in laboratory zebrafish co-housed with zebrafish from the pet trade is associated with RMBV infection. (A) Diagram of cohousing experiment. Tanks were seeded with adult zebrafish from the laboratory (control) or zebrafish from the laboratory and the pet trade (co-housed). (B) A representative individual from the control tank. (C) A representative laboratory zebrafish one month after co-housing with zebrafish from the pet trade. Red arrows highlight necrotic lesions. (D) Transmission of microbes from pet trade to co-housed laboratory zebrafish inferred from host and microbe transcript abundances. Three laboratory fish were sampled from each tank. Samples are grouped by tank and tissue. Columns show estimated transcript abundances in tissue samples from individual laboratory zebrafish.