Extended Data Fig. 5. Restoration of ITCH expression in knockout cells reverses SFTPC mislocalisation.

Control and ITCH knockout HeLa cells transfected with HA-ITCH were assessed for relative ITCH mRNA expression (A), GFP-SFTPC localisation (B) and cell surface full-length SFTPC protein by flow cytometry (C&D). Scale bar = 10 μm. Mean ± SEM, n=3 independent repeats (**=p<0.005, one-way ANOVA with post-hoc Tukey tests).