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[Preprint]. 2024 Aug 26:2023.08.29.555174. Originally published 2023 Aug 29. [Version 2] doi: 10.1101/2023.08.29.555174

Fig. 3. Tau dephosphorylation assay.

Fig. 3.

A and B, anti-pTau (S396) Western blots comparing the pTau phosphatase activity of PP2A in the presence of increasing concentrations (slanted triangle) of added inhibitory protein. A, Effect of IER5-N50, IER5-N50-K17E, and FAM122A on Tau dephosphorylation. B, effect of IER5-FL and IER5-FL-K17E on Tau dephosphorylation. C, Plot of phosphatase activity as a function of inhibitor concentration for the five proteins tested, based on densitometry analysis. Data represent mean ± s.d. of three independent replicates. Data are normalized to the control condition without PP2A/B55α or inhibitor proteins. Data points were fitted using a log(inhibitor) vs. response (variable slope, four parameters) least squares fit model in Prism. The curves were used to estimate the IC50 value for each protein.

See also Supplementary Fig. 5.