Figure 10. Energy landscapes of wt C-subunit and F100A mutant in binary complex.
The free energy landscapes (FEL) were generated based on the principal component analysis (PCA) for the wt C-subunit (A) and F100A mutant (B). The H-bonds are shown in the black dash line and the non H-bond in green along with the distance in Angstroms (Å). The probability distribution plots for specific side chain dynamics are also shown; (C) K105 NZ to E107 OE2, (D) K105 NZ to E121 OE2, (E) K105 NZ to N99 OD1, and (F) N99 N to Y156 OH. The probability density function (PDF) is a relative measure of how densely data points are distributed along the x-axis.