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[Preprint]. 2023 Sep 6:2023.08.30.555581. [Version 2] doi: 10.1101/2023.08.30.555581

Fig. 3 – The permeability of human ALI did not increase after infection, as opposed to HCT-8 cell monolayers.

Fig. 3 –

(A) Trans-epithelial electrical resistance (TEER) and Dextran flux measured across hALI of HCT-8 monolayers infected with C. parvum or C. hominis. hALI was infected 3 days after top medium removal. Mean ± S.E. N = 2, n = 4. Kruskal-Wallis test followed by Dunn’s multiple comparison test. * P < 0.05, **** P < 0.0001. (B) Immunostaining of flat mounted hALI transwell culture showed tight junction (TJ; ZO-1, green) and adherens junction (AJ; E-cadherin, green) organization, with or without infection with C. parvum (Vicia villosa lectin, VVL, red). Scale bar = 50 μm. (C) Intercellular junction organization quantification (IJOQ) of hALI and HCT-8 cells infected with C. parvum vs. not infected, for AJ (E-cadherin) and TJ (ZO-1). hALI was infected 3 days after top medium removal. One representative experiment for each cell type is showed. Mean ± S.E. N = 2, n = 6. Mann-Whitney U test. ** P < 0.01, ns = non significant.