Extended Data Table 3: Significance levels of heart rate differences at 21°C, 28°C., and 35°C.

P values were assessed with the Wilcoxon test comparing the heart rate of the mutant embryos to the heart rate of the respective mock-injected control embryos.

Gene_group_editor	Phenotype	p value	Temperature
adprhl1_Cas9	normal	0.049	21 °C
adprhl1_Cas9	normal	0.137	28 °C
adprhl1_Cas9	normal	0.250	35 °C
adprhl1_evoBE4max	normal	0.129	21 °C
adprhl1_evoBE4max	normal	0.042	28 °C
adprhl1_evoBE4max	normal	0.953	35 °C
blzf1_Cas9	normal	0.741	21 °C
blzf1_Cas9	normal	0.984	28 °C
blzf1_Cas9	normal	0.104	35 °C
blzf1_evoBE4max	normal	0.009	21 °C
blzf1_evoBE4max	normal	0.000	28 °C
blzf1_evoBE4max	normal	0.015	35 °C
btbd1_Cas9	normal	0.009	21 °C
btbd1_Cas9	normal	0.033	28 °C
btbd1_Cas9	normal	0.269	35 °C
btbd1_evoBE4max	normal	0.002	21 °C
btbd1_evoBE4max	normal	0.038	28 °C
btbd1_evoBE4max	normal	0.032	35 °C
gpc5a_Cas9	normal	0.053	21 °C
gpc5a_Cas9	normal	0.452	28 °C
gpc5a_Cas9	normal	0.018	35 °C
gse1_Cas9	normal	0.039	21 °C
gse1_Cas9	normal	0.779	28 °C
gse1_Cas9	normal	0.455	35 °C
IRF1_Cas9	normal	0.057	21 °C
IRF1_Cas9	normal	0.374	28 °C
IRF1_Cas9	normal	0.079	35 °C
pcdh17_Cas9	normal	0.710	21 °C
pcdh17_Cas9	normal	0.889	28 °C
pcdh17_Cas9	normal	0.605	35 °C
phka2_Cas9	normal	0.301	21 °C
phka2_Cas9	normal	0.242	28 °C
phka2_Cas9	normal	0.010	35 °C
phka2_evoBE4max	normal	0.383	21 °C
phka2_evoBE4max	normal	0.465	28 °C
phka2_evoBE4max	normal	0.258	35 °C
PTPRD_Cas9	normal	0.852	21 °C
PTPRD_Cas9	normal	0.844	28 °C
PTPRD_Cas9	normal	0.140	35 °C
PTPRD_evoBE4max	normal	0.607	21 °C
PTPRD_evoBE4max	normal	0.105	28 °C
PTPRD_evoBE4max	normal	0.160	35 °C
rrad_Cas9	normal	0.001	21 °C
rrad_Cas9	normal	0.022	28 °C
rrad_Cas9	normal	0.007	35 °C
rrad_evoBE4max	normal	0.058	21 °C
rrad_evoBE4max	normal	0.113	28 °C
rrad_evoBE4max	normal	0.004	35 °C
SEC61A1_Cas9	normal	0.195	21 °C
SEC61A1_Cas9	normal	0.469	28 °C
SEC61A1_Cas9	normal	0.366	35 °C
zrsr2_Cas9	normal	0.008	21 °C
zrsr2_Cas9	normal	0.135	28 °C
zrsr2_Cas9	normal	0.014	35 °C