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[Preprint]. 2023 Nov 2:2023.09.01.555906. Originally published 2023 Sep 2. [Version 2] doi: 10.1101/2023.09.01.555906

Fig. 3. Phenotype proportions in knockout models of cardiac candidate genes.

Fig. 3

(A) Validation workflow encompassing zygotic microinjections using a HdrR (myl7::eGFP; myl7::H2A-mCherry) reporter line, followed by phenotypic classification of embryos with normal developed hearts and embryos with cardiac-specific phenotypes 4 days post fertilization (dpf). (B) Proportion (bars) and counts (values) of cardiac affected and normally developed embryos after CRISPR-Cas9-mediated knockout of indicated candidate genes versus control (mock injection). (C) Independent replication using base editing: phenotypic distribution (proportion/bars and counts/values) resulting from targeted gene inactivation mediated by introducing premature termination codons via the cytosine base editor evoBE4max and a set of distinct guide RNAs targeting the same genes as in (B).