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[Preprint]. 2025 Feb 15:2023.09.01.555994. Originally published 2023 Sep 2. [Version 2] doi: 10.1101/2023.09.01.555994

Figure 9. FRAP analysis of BiP:sfGFP:HDEL recovery in Atlastin mutants.

Figure 9.

(A) Diagram of the recovery of BiP:sfGFP:HDEL in controls and Atlastin mutants with partial loss or complete loss. (B) Representative slices from movies of controls and Atlastin mutants with partial loss or complete loss co-expressing mCD8:RFP and BiP:sfGFP:HDEL pre-bleach, after FRAP and 2 min post-FRAP (Movies S15–19). (C) Mean recovery of mCD8:RFP in control (black squares) and Atlastin mutants with partial loss (empty lime green triangles) and complete loss (green triangles). (D) Mean recovery of BiP:sfGFP:HDEL in control (black squares) and Atlastin mutants with partial loss (empty lime green triangles) and complete loss (green triangles). The table contains the results from the non-linear fit one-phase association of the data for each dataset. The s.e.m. are represented by solid lines and shading: controls (n=10) have black lines with grey shading, Atlastin mutants with partial loss (n=6) have lime green lines and lime green shading, and Atlastin mutants with complete loss (n= 8) have green lines with light green shading.