[Preprint]. 2023 Aug 30:rs.3.rs-3290462. [Version 1] doi: 10.21203/rs.3.rs-3290462/v1

Table 4.

Differential Expression Analysis Between V0v Group 1 and Group 2 WT and evx1;evx2 Mutant Spinal Interneurons.

Gene Symbol	Gene Name	Hurdle Model Fold-Change		ANOVA Fold-Change
Gene Symbol	Gene Name	Mutant 1 vs Mutant 2	WT 1 vs WT 2	Mutant 1 vs Mutant 2	WT 1 vs WT 2
anos1a	anosmin 1a	↑13.57 ^***	↑7.77 ^***	↑3.79 ^***	↑17.10 ^***
chrna2b	cholinergic receptor, nicotinic, alpha 2b (neuronal)	↑10.27 ^***	↑5.75 ^***	↑83.25 ^***	↑45.56 ^***
fndc4b	fibronectin type III domain containing 4b	↑7.96 ^***	↑2.29 ^***	↑33.76 ^***	↑24.27 ^***
plpp4	phospholipid phosphatase 4	N.C.	↑1.67 ^***	↑54.02 ^***	↑31.39 ^***
cnih3	cornichon family AMPA receptor auxiliary protein 3	↑2.58 ^***	↑1.56 ^***	↑24.54 ^***	↑19.16 ^***
drd2b	dopamine receptor D2b	N.C.	↑1.41 ^***	↑30.07 ^***	↑19.48 ^***
esrrb	estrogen-related receptor beta	↓5.49 ^***	↓72.83 ^***	↓16.07 ^***	↓17.79 ^***
scxa	scleraxis bHLH transcription factor a	↓1.92 ^***	↓25.39 ^***	↓3.54 ^***	↓4.53 ^***
svild	supervillin d	↓1.35 ^***	↓1.62 ^***	↓7.24 ^***	↓6.94 ^***

Gene-specific analyses of differential expression, created through Hurdle model (columns 3–4) and ANOVA (columns 5–6) statistical comparisons between distinct cell clusters in our 48 h evx1^i232/+;evx2^sa140/+ heterozygote incross single-cell atlas (see Fig 7A and also Methods for experimental details and rationale for using both statistical methods). For these comparisons, the Hurdle model is probably the most statistically robust method as there are sufficient cell numbers in each group to effectively model the variance (see Methods for more information). We also provide the ANOVA data for completeness and comparison. Columns 1 and 2 show the gene symbol and the full gene name respectively. Columns 3–6 show fold-change values. = fold-change increase, ^– = fold-change decrease in the antecedent (first) population compared to the consequent (second) population in each comparison. Statistically significant (P<0.05) values are indicated in bold.

^***

P<0.001.

N.C. = Not Calculated. Differential expression cannot be calculated, usually because there is no expression in one population in the comparison. Mutant Group 1 is the antecedent population for columns 3 and 5 respectively. WT Group 1 is the antecedent population for columns 4 and 6 respectively Mutant Group 2 is the consequent population for columns 3 and 5 respectively. WT Group 2 is the consequent population for columns 4 and 6 respectively. Additional data for each comparison are available in Supp. Data Tables 2 (Hurdle model data) and 3 (ANOVA data).