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. 2023 Aug 28;19(8):e1011616. doi: 10.1371/journal.ppat.1011616

Fig 1. DENV-specific IgG, but not IgA, mediates ADE in U937 cells.

Fig 1

A) Quantification of FcγRIIa (CD32), FcγRI (CD64), FcαR (CD89), and DC-SIGN (CD209) expression on U937 cells by flow cytometry. Histogram labels are isotype control-subtracted geometric mean fluorescence intensity (MFI) values. Dashed line indicates isotype control staining. B) Assessment of DENV infection-enhancing activity of DENV-specific IgG and IgA in U937 cells. Data shown as fold change in U937 cell infection frequency relative to DENV alone at the indicated antibody concentration. Dashed line indicates infection rate observed with virus alone, set to a value of 1 for each biological replicate. C) The area under the ADE curves for each of 6 independent biological replicates of IgG and IgA infection experiments relative to infection achieved with DENV alone. * p < 0.05, Wilcoxon matched-pairs test.