Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 11;42(37):2737–2750. doi: 10.1038/s41388-023-02781-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Western blot assays on the level of phosphorylated STAT3 in total protein extracts from BMDMs (the up panel) or M0-like THP-1 cells (the down panel). b The expression levels of STAT3 and p-STAT3^Y705 were detected in total protein extracted from BMDMs (the up panel) or M0-like THP-1 cells (the down panel) after 6 h stimulation by tumor-CM. c Co-IP assay on the expression of STAT3 protein and JMJD6 protein in TAM-like THP-1 cells. d GST pull-down assays on JMJD6-His and STAT3-GST. e Western blot assays on the purified recombinant STAT3 proteins (1–7.5 μg/lane) were incubated with purified recombinant JMJD6 proteins (1–7.5 μg/lane) in an in vitro phosphorylation system. f Inhibitory effect of napabucasin on phosphorylation of Y705 residue of STAT3 protein in macrophages. g Napabucasin inhibited the production of IL-10 protein in tumor-CM-stimulated macrophages. h Napabucasin inhibited the secretion of IL-10 in macrophage supernatant. Data represent mean ± SD (n = 3). ns: no significant difference, ***P < 0.001.