Fig. 2. NfP2X7-CAR-T cells consist of mostly naive and central memory phenotypes.

a Frequency of EGFR reporter expression on total CD3⁺ cells 7 days post-transduction (n = 13), ratio of CD8⁺/CD4⁺ of CD3⁺ end of expansion on d14 post-PBMC; n = 13. Data is pooled from 13 independent experiments each dot represents an experiment conducted with T cells from a separate donor. b Representative FACS plots for CD45RA⁺, CD62L⁺, and CCR7⁺ expression of CD4⁺ and CD8⁺ nfP2X7-M CAR-T cells at end of expansion on d14 post-PBMC. c Frequency of T_SCM, T_Naive, T_CM, T_EM and T_EMRA and populations based on CD45RA⁺, CD62L⁺ and CCR7⁺ expression; n = 7. Pooled data from seven independent experiments. Data represents mean ± SEM. Statistical significance was evaluated by paired T test. ns: non-significant.