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. 2023 Sep 1;4(3):102536. doi: 10.1016/j.xpro.2023.102536

Figure 6.

Figure 6

Expected performance of MagReSyn Ti-IMAC HP and Zr-IMAC HP for TMT-based analysis from bulk HeLa lysate

(A) Comparison between MagReSyn Ti-IMAC-HP and Zr-IMAC-HP beads for phosphopeptide enrichment. The data was acquired in DDA mode with 15k MS2 resolution and, using a 60SPD Evosep gradient for all amounts. The identifications represents the median of 3 replicates per conditions, the error-bars represent the standard deviation.

(B) Barplot showing the phosphopeptide multiplicity as a function of the peptide input amount for both Ti-IMAC-HP and Zr-IMAC-HP. The multiplicity corresponds to the percentage of multiphosphorylated peptides.

(C) Boxplot displaying the measured phosphopeptides intensity as a function of the peptide input amount for both Ti-IMAC-HP and Zr-IMAC-HP beads.