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. 2023 Sep 1;4(3):102536. doi: 10.1016/j.xpro.2023.102536

Figure 7.

Figure 7

Expected performance of column and IMAC bead type DIA based phosphopeptide analysis using WHISPER 40SPD Evosep gradient

(A) Comparative bar plot assessing the effect of the column type (Evosep (EV1112) vs. IonOpticks (AUR3-15075C18-TS)) on the number of phosphopeptides identified when using 2.5 μg of peptide input for phosphopeptide enrichment with MagReSyn Ti-IMAC-HP beads with the 40 SPD gradient (n = 3).

(B) Comparative bar plot assessing the effect of the beads type (Ti-IMAC-HP vs. Zr-IMAC-HP) on the number of phosphopeptides (class I) identified when using 2.5 μg of peptide input for phosphopeptide enrichment using an IonOpticks column (n = 3) for phosphopeptide separation. Adapted from Martinez-Val et al.2