Table 1.
Plates with their contents used for the automated PAC procedure on KingFisher Flex or Apex models
| KingFisher 96 deep-well plate order | |||
|---|---|---|---|
| Plate number and name | μL/ well | Buffer | Time per step (min) |
| 1: Tip plate | |||
| 2: MagReSyn Hydroxyl, Sample lysate, Bind solvent | Varies based on sample volume | 70% MeCN final | 20 |
| 3: Wash | 500 | Wash buffer 1 | 2.5 |
| 4: Wash | 500 | Wash buffer 1 | 2.5 |
| 5: Wash | 500 | Wash buffer 1 | 2.5 |
| 6: Wash | 500 | Wash buffer 2 | 2.5 |
| 7: Wash | 500 | Wash buffer 2 | 2.5 |
| 8: Digest | 200–300 | Digest buffer | 120–960 |