Table 2.

Plates with their contents used for the automated PAC procedure on KingFisher Duo and Presto models

KingFisher 96 deep-well plate order
Plate row number and name	μL/ well	Buffer	Time per step (min)
H: Tip plate
G: MagReSyn Hydroxyl, sample lysate, bind solvent	Varies based on sample volume	70% MeCN final	20
F: Wash	500	Wash buffer 1	1
E: Wash	500	Wash buffer 1	1
D: Wash	500	Wash buffer 1	1
C: Wash	500	Wash buffer 2	1
B: Wash	500	Wash buffer 2	1
A: Digest	200–300	Digest buffer	240–960