Table 3.
Lysate amount (μg) | Lysate volume (μL) | Bead amount (μg) | Bead volume (μL) | Bind solvent volume (μL) (100% MeCN) |
---|---|---|---|---|
5 | 40 | 200 | 10 | 92 |
10 | 40 | 200 | 10 | 92 |
20 | 40 | 200 | 10 | 92 |
50 | 100 | 200 | 10 | 230 |
100 | 200 | 200 | 10 | 460 |
200 | 300 | 400 | 20 | 700 |
1000 | 300 | 2000 | 100 | 700 |