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Recommended peptide to bead ratios for phosphopeptide enrichment
| Digest amount (μg) | Peptide : Bead ratio | Bead amount (μg) | Bead volume (μL) |
|---|---|---|---|
| 2.5 | 1:40 | 100 | 5 |
| 10 | 1:10 | 100 | 5 |
| 20 | 1:5 | 100 | 5 |
| 50 | 1:2 | 100 | 5 |
| 100 | 1:2 | 200 | 10 |
| 200 | 1:2 | 400 | 20 |
| 500 | 1:2 | 1000 | 50 |
| 1000 | 1:2 | 2000 | 100 |
