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. 2023 Sep 1;4(3):102536. doi: 10.1016/j.xpro.2023.102536

Table 8.

Reference table for Evosep gradients and column set-up

Gradient Column Emitter Input (into column)
30SPD Evosep endurance column (15 cm × 150 μm, 1.9 μm beads, EV1106) Stainless-steel emitter (30 μm i.d., Evosep, EV1086) >500 ng tryptic peptides or 200 μg of enriched phosphopeptides
60SPD Evosep performance column (8 cm × 150 μm, 1.5 μm beads, EV1109) Stainless-steel emitter (30 μm i.d., Evosep, EV1086) >100 ng tryptic peptides or 100 μg of enriched phosphopeptides
20 SPD IonOpticks Aurora column (15 cm × 75 μm, 1.7 μm cat#AUR3-15075C18-TS) or Evosep performance column (15 cm × 75 μm, 1.9 μm beads, EV1112) Fused silica emitter (10 μm i.d., Evosep, EV1087). Only for Evosep column. <50 ng tryptic peptides or <50 μg of enriched phosphopeptides
40SPD IonOpticks Aurora column (15 cm × 75 μm, 1.7 μm cat#AUR3-15075C18-TS) or Evosep performance column (15 cm × 75 μm, 1.9 μm beads, EV1112) Fused silica emitter (10 μm i.d., Evosep, EV1087). Only for Evosep column. <50 ng tryptic peptides or <50 μg of enriched phosphopeptides