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Expected number of phosphopeptides quantified using offline fractionation TMT-based approaches
| Input amount (μg digest/sample) | Stage-tip fractionation (STF) | Microflow fractionation (MF) | LFQ DIA |
|---|---|---|---|
| 12.5 | 9856 | 13250 | 7000–9000 |
| 2.5 | 5485 | 4684 | |
| 1 | 4577 | 4342 |
