| Reagent | Final concentration | Amount (555 mL) |
|---|---|---|
| Dulbecco’s modified Eagle medium (DMEM) | N/A | 500 mL |
| Penicillin /streptomycin | 1% (v/v) | 5 mL |
| Fetal bovine serum | 10% (v/v) | 50 mL |
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