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. 2023 Sep 1;4(3):102536. doi: 10.1016/j.xpro.2023.102536
Reagent Final concentration Amount (20 mL)
MeCN 80% (v/v) 16 mL
TFA 1% (v/v) aqueous solution 200 μL
MilliQ water 3.8 mL
Prepare freshly before each use.
∼0.5 mL of Phosphopeptide Wash buffer 1 is needed for each sample