Reagent | Final concentration | Amount (20 mL) |
---|---|---|
MeCN | 80% (v/v) | 16 mL |
TFA | 1% (v/v) aqueous solution | 200 μL |
MilliQ water | 3.8 mL | |
Prepare freshly before each use. ∼0.5 mL of Phosphopeptide Wash buffer 1 is needed for each sample |
Reagent | Final concentration | Amount (20 mL) |
---|---|---|
MeCN | 80% (v/v) | 16 mL |
TFA | 1% (v/v) aqueous solution | 200 μL |
MilliQ water | 3.8 mL | |
Prepare freshly before each use. ∼0.5 mL of Phosphopeptide Wash buffer 1 is needed for each sample |