Reagent	Final concentration	Amount (10 mL)
SDS (20% diluted in MilliQ water)	5% (w/v)	2,5 mL
Tris/HCL pH 8.5 (1 M)	100 mM	1 mL
Tris (2-carboxyethyl) phosphine (TCEP, 0.5 M diluted in MilliQ water))	5 mM	100 μL
Chloroacetamide (CAA, 550 mM diluted in 25 mM AmmBic))	10 mM	180 μL
MilliQ water	N/A	6.72 mL