Reagent	Final concentration	Amount (15 mL)
AmmBic (1 M, pH 8.5)	50 mM	750 μL
MilliQ water		14.250 mL
Lys-C (0.1 μg/μL)	1 μg per 100 μg protein (1:100 ratio). For > 150 μg protein use 1:500 ratio	10 μL per 100 μg of protein
Trypsin (0.1 μg/μL)	2 μg per 100 μg protein (1:50 ratio). For > 150 μg protein use 1:250 ratio	20 μL per 100 μg of protein
Prepare freshly before each use. ∼ 300 μL PAC digestion buffer is needed for each sample