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. 2023 Aug 2;129(6):925–934. doi: 10.1038/s41416-023-02369-w

Fig. 5. Co-expression of CD80 on sEVs disrupted PD-L1-positive sEV-mediated PD-1 binding and T cell inhibition.

Fig. 5

a Quantification for the MFI of CD80 and PD-1 on sEVs derived from human cell lines, the MFI is tested by high-resolution flow cytometry. b The percentages of co-expressing CD80 and PD-L1 sEVs derived from human cell lines. c Western blot analysis of CD80 and PD-L1 on sEVs derived from CD80 and/or PD-L1-expressing 293E cells. All lanes were loaded with the same amount of total protein. d The proportions of PD-1-binding of sEVs treated with indicated sEVs. e Representative contour plots of T cells (stimulated with PHA) after incubation with indicated sEVs examined for the expression of Ki-67. The proportions of Ki-67 positive T cells are shown in the right panel. Data are mean ± SD of three independent experiments. P values are from one-way ANOVA test.