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. 2023 Aug 21;30:534–545. doi: 10.1016/j.omtm.2023.08.012

Figure 1.

Figure 1

Dual-AAV8(Y733F)-MYO7A vectors restore MYO7A expression and prolong the survival of shaker-1 cochlear hair cells

(A) Representative confocal images of inner hair cells (IHCs) and outer hair cells (OHCs) of the cochlear middle turn. In an untreated wild-type mouse, MYO7A expression (green) is found in the IHCs and OHCs. The shaker-1 mouse does not express MYO7A. Shaker-1 mice that were treated with dual-AAV8(Y733F)-MYO7A vectors exhibit restoration of MYO7A in transduced IHCs and OHCs. Images were taken at P30–P37. The scale bar represents 20 μm. (B and C) Quantification of IHC (B) and OHC (C) transduction rates in shaker-1 mice that were treated with dual-AAV8(Y733F)-MYO7A vectors. (D and E) Quantification of IHC (D) and OHC (E) survival in shaker-1 mice that were treated with dual-AAV8(Y733F)-MYO7A vectors. Shaker-1 mice that were treated with dual-AAV8(Y733F)-MYO7A vectors showed significant improvement in hair cell survival compared with untreated shaker-1 mice. n = the number of animals used. Error bars represent standard errors. ∗∗p < 0.01, ∗∗∗∗p < 0.0001.