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. 2023 Sep 8;13:14790. doi: 10.1038/s41598-023-42046-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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RT-qPCR validation of DE genes in NvGcm-depleted larvae. (a) Total number of DE genes identified in RNA-seq at p-adj < 0.2, numbers of DE genes selected for RT-qPCR validation, and numbers of genes validated by RT-qPCR. Functional classification of RT-qPCR validated DE genes (b) NvGcm KD efficiency in experimental batches used for RT-qPCR validation. Each experiment is color-coded. (c) Relative mRNA expression of validated candidate genes in NvGcm-depleted larvae compared to controls. Some up-regulated DE genes (NvPla2.1, NvEts-related, and NvMpeg1) are also shown. Each data point is color-coded and corresponds to the experiment in b. Simplified cartoons to the left of the bar graphs in c demonstrate cellular processes in which validated candidate genes are involved.