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. 2023 Sep 8;13:14790. doi: 10.1038/s41598-023-42046-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology of NvEAAT1-positive cells. (a) EAAT1-expressing cells stained with German Research Products (rabbit) and Bio-Techne (sheep) anti-EAAT1 antibodies are broadly detected throughout the aboral region of the body column during the juvenile polyp stage (9dpf). Cells have flat morphology and lack processes. (b) Expression of NvEAAT1 at different developmental stages revealed by immunostaining with German Research Products anti-EAAT1 antibody. EAAT1-expressing cells became visible during larval stages. Side views of (from left to right): late gastrula stage (3dpf), early planula stage (4dpf), planula stage (5dpf), tentacle bud stage (6dpf). Asterisks indicate oral positions. Large and small scale bars are 100 μm and 10 μm, respectively.