Fig. 3.

Representative images of basal protein expression levels (Western Blot) for all proteins studied and total amount of protein loaded (Reactive Brown Staining) from one woman and four men participating in the study. A control human sample (non-experimental) was included onto each gel in triplicate or quadruplicate to allow normalization and as a loading control. Images from top to bottom: SERCA1, SERCA2, phosphofructokinase1, calsequestrin1, calsequestrin2, citrate synthase, ATP5A, UQCRC2, SDHB, COXII, NDUFB8, myoglobin and Reactive Brown (as total protein loading control) with experimental samples run in duplicate (Panel A), and Nrf2, pSer⁴⁰ Nrf2, Keap1, catalase, SOD1, SOD2 and Reactive Brown (Panel B). CON, control non-experimental sample; M, sample indicating a male participant; W, sample indicating a female subject. Estimated molecular weights are indicated on the right side of the blot.