Fig. 4.
Metrnl prevents cardiomyocyte injury upon hyperglycemic stimulation in cardiomyocytes. Cardiomyocytes were pre-incubated with various doses of Metrnl for 30 min followed by incubation of HG for additional 72 h. (A) LDH release was determined. (B) Cell viability. (C) Relative EdU-positive cells. (D) Representative photographs of EdU staining. Scale bar = 100 μm. (E) Representative photographs of α-actinin staining. Scale bar = 20 μm. (F) Relative mRNA levels of ANP, BNP, and β-MHC. (G) Representative photographs of TUNEL staining. Scale bar = 100 μm. (H) Caspase-3 activity. (I) Relative mRNA levels of Bax and Bcl-2. (J) Representative photographs of DHE staining. Scale bar = 150 μm. (K) MDA content. (L) Relative mRNA levels of p47phox and p67phox. n = 4–6. *P < 0.05 versus 0 or NG, †P < 0.05 versus HG.