Figure 4.

Immunophenotyping of murine B cells after in vivo vector administration

(A) Gating strategy to define individual B cell populations. All populations were initially gated as singlets/live/CD19+/CD3–. GC, germinal center; FO, follicular; MZ, marginal zone; CD71hi, proliferating; PBs, plasmablasts. (B) eGFP expression in defined cell populations. Statistical analysis was carried out using Ad5.CMV as a representative group using ordinary one-way ANOVA with Tukey’s multiple comparisons test on log transformed data. (C) Fold change in eGFP expressing cells in individual B cell populations compared with all B cells. GC and PB subsets were subjected to analysis via ordinary one-way ANOVA with Tukey’s multiple comparisons test. (D) Representative analysis of cell marker expression in eGFP+ and eGFP– cells. In all cases three mice per group were injected with 5 × 10¹⁰ vp as described previously. All data are expressed as means ± SD and normalized to a PBS mock control.