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. 2023 Jul 5;31(9):2734–2754. doi: 10.1016/j.ymthe.2023.06.016

Figure 5.

Figure 5

Rescue of STAT1 expression in conditional KO mice or KO cell restored renal damage

(A) P-STAT1/STAT1, PP65/P65, KIM1 protein level was determined by western blot. (B) Relative mRNA levels of STAT1, KIM1 and proinflammatory genes. (C) Representative PAS staining of kidney in lentivirus-mediated STAT1 overexpression in mice treated with cisplatin. (D, E) Serum creatinine and BUN levels. (F) IF staining of KIM1, F4/80, and TNF-α. (G) Protein levels of P-STAT1/STAT1, PP65/P65, and quantification. (H) Relative mRNA levels of STAT1, KIM1, and proinflammatory indexes were determined by real-time PCR. (I) IF staining of P-STAT1 in TECs. Data represented the mean ± SEM for six mice. ∗p < 0.05, ∗∗∗p < 0.001 vs. FF + Cis. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. KspCre+Cis. Independent experiments were performed throughout the in vitro studies in triplicate. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs. WT + Cis. #p < 0.05, ###p < 0.001 vs. KO + Cis. Cis, cisplatin; WT, wild type.