Figure 3.

CR2-Crry inhibits astrocytopathy, demyelination, and inflammation ex vivo

(A) Schematic diagram and timeline for experimental procedures of NMO cerebellar slice culture model with CR2-Crry or PBS (vehicle) treatment. (B) Representative immunostaining images of GFAP (upper, green) and AQP4 (lower, red) in cerebellar slices of the four groups. Scale bars, 100 μm. (C) Quantification of GFAP (left) and AQP4 (middle) relative immunofluorescence intensity and NMO-like lesion score (right) in cerebellar slices of the four groups. n = 5 or 6 slices (3 sections/slice) per group. (D) Representative immunostaining images of MBP (upper, red) and NFH (lower, green) in cerebellar slices of the four groups. Scale bars, 100 μm. (E) Quantification of MBP (left) and NFH (right) relative immunofluorescence intensity in cerebellar slices of the four groups. n = 5 slices (3 sections/slice) per group. (F) Representative immunostaining images of C3d (upper, green) and C5b-9 (lower, red) in cerebellar slices of the four groups. Scale bars, 100 μm. (G) Quantification of C3d (left) and C5b-9 (right) relative immunofluorescence intensity in cerebellar slices of the four groups. n = 5 slices (3 sections/slice) per group. (H) Representative immunostaining images of Iba1 (upper, green) and CD68 (lower, red) in cerebellar slices of the four groups. Scale bars, 100 μm. (I) Quantification of Iba1⁺ microglia (per mm³) (left) and CD68⁺ Iba1⁺ microglia percentage (right) in cerebellar slices of the four groups. n = 5 slices (3 sections/slice) per group. All data are presented as mean ± SEM. Statistical analysis was performed with one-way ANOVA and Tukey’s post hoc multiple comparisons. Non-significant comparisons are not identified. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.