Table 1.

Lipid-lowering effect of 89 clinical prescriptions used frequently on HepG2 cell lines

NO.	Prescriptions	Prevention strategy	Treatment strategy
		Reduction rate (%)	Reduction rate (%)
1	Antae-eum	-9	57
2	Baenongsangeup-san	30	42
3	Bangkeehwangkee-tang	-12	47
4	Banhabakchulcheonma-tang	40	33
5	Banhahoo Granule	24	26
6	Banhahubak-tang	28	53
7	Banhasasim-tang	-28	21
8	Bojungikgi-tang	19	22
9	Buhnsimgieum	19	35
10	Bulhwangeumjeonggi-san	15	39
11	Chengsimyeunjaeum	-3	27
12	Cheongsangbangpung-san	49	52
13	Cheongsanggyeontong-tang	34	51
14	Cheonwangbosim-dan	-15	34
15	Chokyungjongok-tang	32	44
16	Chungseoikgi-tang	36	29
17	Daegunjoong-tang	28	50
18	Daehwajung-eum	29	30
19	Daehwangmokdan-tang	27	18
20	Daeshiho-tang	27	35
21	Daeyoung-jeon	18	28
22	Dangguijakyak-san	17	47
23	Dangguiniantong-tang	30	3
24	Dangguisu-san	-18	14
25	Doinseunggi-tang	28	19
26	Dokhwalgisaeng-tang	28	43
27	Eunkyo-san	19	37
28	Galgeun-tang	17	5
29	Gamiondam-tang	24	45
30	Gamisoyo-san	13	10
31	Gumiganghwal-tang	17	19
32	Gungha-tang	17	36
33	Gwakhyangjeonggi-san	-13	-1
34	Gyejibokryeong-hwan	-20	-2
35	Hwanggigunjung-tang	39	28
36	Hwanglyeonhaedok-tang	11	56
37	Hyangsapyeongwi-san	24	16
38	Hyangsayukgunja-tang	-21	16
39	Hyangso-san	15	19
40	Hyeonggaeyeongyo-tang	28	43
41	Hyulbuchukeo-tang	25	48
42	Insamyangyoung-tang	23	51
43	Jaeumganghwa-tang	27	33
44	Jagamcho-tang	32	42
45	Jakyak gamcho-tang	6	12
46	Jihwangeum-ja	-16	53
47	Jodeung-san	25	56
48	Kamiguibitang	26	-3
49	Kumikanghal-tang	10	24
50	Kungkwikyoae-tang	29	11
51	Kyeji-tang	11	24
52	Maekmundong-tang	-41	-3
53	Mahwang-tang	6	27
54	Majain-hwan	-9	29
55	Naeso-san	17	16
56	Ojeok-san	40	33
57	Oncheong-eum	33	53
58	Onkyung-tang	2	-10
59	Oryeong-san	36	26
60	Paljung-san	10	44
61	Palmijihwang-tang	-17	15
62	Palmul-tang	41	27
63	Pyeongwi-san	40	16
64	Saengkangsasim-tang	36	34
65	Saengmaek-san	3	0
66	Samchulgeonbi-tang	31	24
67	Samryeongbaekchul-san	-23	-12
68	Samul-tang	23	15
69	Sanjoin-tang	28	50
70	Sayeoksan	5	23
71	Sikyungbanha-tang	35	28
72	Sipjeondaebo-tang	25	42
73	Siryung-tang	30	51
74	Socheongryong-tang	14	17
75	Sogunjung-tang	28	44
76	Sokyunghwalhul-tang	20	52
77	Sopung-san	26	46
78	Ssanghwa-tang	28	39
79	Taglisodog-eum	34	43
80	Ukgan-sangajinphibanha	31	45
81	Wiryeong-tang	38	30
82	Yeongyechulgam-tang	-2	18
83	Yeonkyopaedok-san	24	35
84	Yijung-tang	38	35
85	Yongdamsagan-tang	17	28
86	Youngseonjaetong-eum	23	52
87	Yukgunja-tang	-12	55
88	Yukmijihwang-tang	40	30
89	Zeolyung-tang	38	22
-	Atorvastatin (20 µM)	32	49

HepG2 cells were cultured in 96-well plates at a density of 5 × 10⁴ cells/well for 24 h. To investigate the preventive effect, cells were incubated with each of the above prescriptions (100 µg/mL) or atorvastatin (20 µM) as a positive control in the presence of a free fatty acid (FFA) mixture (oleic acid/palmitic acid, 2:1 ratio) for 24 h. The following day, the intracellular lipid content was fluorescently measured using AdipoRed reagent at excitation/emission wavelengths of 485/572 nm. For the treatment effect experiment, lipid accumulation was induced in the cells with an FFA mixture for 24 h and then the cells were incubated with each prescription (100 µg/mL) or atorvastatin (20 µM) for a further 24 h. The lipid content was analyzed using AdipoRed reagent according to the manufacturer’s protocol.