. Author manuscript; available in PMC: 2024 Sep 1.

Published in final edited form as: Comp Biochem Physiol A Mol Integr Physiol. 2023 May 23;283:111453. doi: 10.1016/j.cbpa.2023.111453

Table 1.

Primer sequence for qPCR Assays

Target	Forward Primer	Reverse Primer	Efficiency

DBH	CCGATGATGTCCTGACAATGGA	TCCGTGATGTAGCACCAGTAAG	104%
vGluT2	GATCGTCGGAGCCATGACTAAG	CGGAGGCAAATAAGCCGTAGAAG	102%
GAD1	CAGACCAGGCTCGTTTCCTA	CCGCCCTGGAGATAGTCTTTC	100%
TASK2	CAGGACAAGGAAGCCACGATA	GGTCTCCCAGGGTTCAGATTC	100%
ASIC2	GTGCAGAACCAGCGCTAAG	CAGGGCATTGTACACATGCA	100%
Kir5.1	ACGGCAAACTGTGCCTCATG	CGCACGTTTCCTTCAACAACA	99%
Target	Probe Sequence	Probe-Quencher	NCBI accession # Lithobates catesbeianus

DBH	TCTTGCTCCAGATGTTGTCATTCCAGA	FAM-BHQ1a	PIO33490.1
vGluT2	CACGTGAGGAATGGCAATATGTCTTCC	HEX-BHQ1a	PIO31042.1
GAD1	CCACTCTTGCCAGACTAGCCTCCTT	FAM-BHQ1a	PIO41232.1
TASK2	TCATCAACCAATTAGACCGGATCAGTGA	HEX-BHQ1a	PIO29936.1
ASIC2	TTTACTGACAGAGAAGGATGGAGGGTTT	Texas Red-BHQ2a	PIO37540.1
Kir5.1	TGGCGTGTTGGTGACTTTCGAC	FAM-BHQ1a	XM_040331031.1 ^*

Kir 5.1 coding sequence was found in Rana temporaria (closely related to L. catesbeianus); see methods.