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. 2023 Sep 9;24:29. doi: 10.1186/s12865-023-00565-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Migratory conventional dendritic cells in mesenteric lymph nodes express an activated phenotype upon live soil exposure. Spleen and mesenteric lymph nodes were collected at day 21 and myeloid cell compartments were analyzed using flow cytometry. Conventional dendritic cells 1 (cDC1) and 2 (cDC2) were defined as XCR1⁺SIRPα⁻ or XCR1⁻SIRPα⁺, respectively, and quantified from the from the CD11c^Int/HiMHCII^Hi population (A). The percentage of cDC1 and cDC2 showed no differences between mouse groups. (B) CD80 expression on cDC1 and cDC2 was quantified using median fluorescence intensity as a measure for activation. Data are shown as scatter plots with mean and 95% CI (n = 8 per group, from two independent exposure experiments). Each symbol represents an individual mouse, lines indicate the mean. The data was analyzed with One-way ANOVA with Tukey´s multiple comparison test, *p < 0.05