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. 2023 Sep 9;24:29. doi: 10.1186/s12865-023-00565-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Exposure to autoclaved soil downregulates IgM in B1 cells from spleen while live soil upregulates CD8⁺T cells in mesenteric lymph nodes. Spleen and mesenteric lymph nodes were collected at day 21 and lymphoid cell compartments were analyzed using flow cytometry. (A) T cells (CD3⁺), helper T cells (CD3⁺ CD4⁺), cytotoxic T cells (CD3⁺ CD8⁺) and B cells (CD19⁺) in the mLN. (B) The percentage of CD69 + effector cytotoxic T cells (CD8⁺ CD44⁺ CD62L⁻) in the mLN. (C) CD3⁺, CD4⁺, CD8⁺ and CD19⁺ cells in spleen. (D) B1 cells (CD19⁺ B220⁻) and IgM expression on B1 cells in spleen. MFI was measured from total B1 population. (E) B2 cells (CD19⁺ B220⁺) and IgM expression on B2 cells in spleen. MFI was measured from total B2 population. Data are shown as scatter plots with mean and 95% CI (n = 8 per group, from two independent exposure experiments). Each symbol represents an individual mouse, lines indicate the mean. The data was analyzed with One-way ANOVA with Tukey´s multiple comparison test, *p < 0.05