Multiplex PCR of reference strains. DNA from reference strains was used as template DNA for PCR. Products were separated on a 3% agarose gel and compared to a positive control. Bands corresponding to Stx1, eaeA, F41, K99, STa, and Stx2 are indicated. Lane A, strain B41; lane B, strain B44; lane C, strain 431; lane D, strain 637; lane E, strain 3081; lane F, strain 933; lane G, strain CHMC6; lane H, strain E2348-69; lane I, RDEC-1.